Desorption Electrospray Ionization (DESI) mass spectrometry imaging of the distribution of rohitukine, in the seedling of Dysoxylum binectariferum Hook.F

Mohana Kumara P, Srimany A, Arunan S, Ravikanth G, Uma Shaanker R, Pradeep T (2016) Desorption Electrospray Ionization (DESI) Mass Spectrometric Imaging of the Distribution of Rohitukine in the Seedling of Dysoxylum binectariferum Hook. F. PLoS ONE 11(6): e0158099.
Mohana Kumara P; Srimany A; Arunan S, Ravikanth G; Uma Shaanker R and Pradeep T
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11(6): June 30, 2016
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Ambient ionization mass spectrometric imaging of all parts of the seedling of Dysoxylum binectariferum Hook. f (Meliaceae) was performed to reconstruct the molecular distribution of rohitukine (Rh) and related compounds. The species accumulates Rh, a prominent chromone alkaloid, in its seeds, fruits, and stem bark. Rh possesses anti-inflammatory, anti-cancer, and immuno-modulatory properties. Desorption electrospray ionization mass spectrometry imaging (DESI MSI) and electrospray ionization (ESI) tandem mass spectrometry (MS/MS) analysis detected Rh as well as its glycosylated, acetylated, oxidized, and methoxylated analogues. Rh was predominantly distributed in the main roots, collar region of the stem, and young leaves. In the stem and roots, Rh was primarily restricted to the cortex region. The identities of the metabolites were assigned based on both the fragmentation patterns and exact mass analyses. We discuss these results, with specific reference to the possible pathways of Rh biosynthesis and translocation during seedling development in Dbinectariferum

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Dr. G Ravikanth
Dr R Uma Shaanker
Biotechnological Interventions for conservation and utilization of forest resources. Funded by Department of Biotechnology (DBT) New Delhi.
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